User Overview

Followers and Following



Total Followers - Last Year
Daily Follower Change - Last Year
Daily Tweets - Last Year

Tweet Stats

Analysed 15,901 tweets, tweets from the last 194 weeks.
Tweets Day of Week (UTC)
Tweets Hour of Day (UTC)
Tweets Day and Hour Heatmap (UTC)


Last 50 tweets from @Addgene
Depositor: Sachdev Sidhu
Purpose: Protein expression and purification of human SH3 domain construct ARHGEF16-1/1
This image was created with bacteria expressing a fluorescent protein and blue light! The optogenetic tool behind this image was developed from the labs of @barbara_synbio and @KhammashLab and can be used to control gene expression with light.
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
Addgene Retweeted ·  
ALERT! ALERT! Anti-FLAG frankenbody on the loose!
Believed to have escaped on FLAG day 6/14/2021. Description follows. Spread the word!
See also:…
A great article about Fast Grants which quickly funded research from many labs on COVID-19 (including Addgene 🙏). Now, @patrickc @tylercowen @pdhsu highlight some of the research as a result of Fast Grants and what they learned about funding in science.
Addgene Retweeted ·  
Very happy to share our collaboration demonstrating how axolotls regenerate damaged spinal cord through synchronous activation of stem cells residing within 800 um of the injury. [1/8, a thread]
Press release (IMP):….
Addgene Retweeted ·  
Looking at this image gives me immense pleasure, I admit
😋 I am sure Mustafa feels the same way
Addgene Retweeted ·  
Check out the latest @Addgene blog post for a feature on the new optogenetic tool developed by #CIBSS investigator Barbara Di Ventura @barbara_synbio and collaborator @KhammashLab.
🎶 Complete with lyrics for their #musicalabstract “Bye bye L-arabinose drive...
Addgene Retweeted ·  
Happy Transposon Day 2021!

On June 16 in 1902, Barbara McClintock was born, founder of the #transposon field and Nobel laureate. We, the editors of @MobDNAjournal, are curious to hear how she or her work influenced your career! #TransposonDay2021…
Q: What’s the smallest unit of an antibody molecule that can bind an antigen?
A: An scFv.

Check out our latest blog post from @ElizabethKenkel to learn more about scFvs!
Antibodies 101: Single Chain Fragment Variables (scFvs)
New codon-optimized fluorescent protein plasmids from @PatrickDijck's for Candida albicans:
New plasmids from Hideji Murakoshi: Photoactivatable CaMKII induces synaptic plasticity in single synapses.
Depositor: Ginkgo Bioworks @Ginkgo
Purpose: Yeast Expression plasmid for #SARS-CoV-2 ORF8 protein
🔬⚛️🐛 What's your favorite molecule, model organism, or reagent?

We had fun in one of the team meetings and decided to share our favorite molecule, reagent, and model organisms as our meeting backgrounds, so we thought we'd ask you the same question! #SciComm #ScienceTwitter
Addgene Retweeted ·  
Here is a new protocol to generate versatile ss-dsDNA hybrid substrates for @LUMICKS_nl C-Trap and optical tweezers in general.

A cool collaboration with the @BoultonLab

@ImperialMed @MRC_LMS @TheCrick…
Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis
Addgene Retweeted ·  
Scientific question: when wanting to evolve an enzyme via error-prone PCR: how many plates would you use after transformation? I read people talk of library sizes of 10^9. How many colonies do I expect on a single plate? So how many plates shall we use to get this library size?
To celebrate the 100,000 plasmids milestone, we made a Giphy account! Find our GIFs by searching "Addgene" in Twitter, etc. and find our stickers on Instagram or Tiktok as well! We'll be adding more in the future and let us know if you have a suggestion!
Addgene GIFs on GIPHY - Be Animated
These plasmids from Emma Rawlin's lab expand our ability to study endogenous gene function in organoids by providing gene manipulation tools for conditional gene knock-down or overexpression, CRISPRi, & CRISPRa adapted for tissue-derived organoids studies
Great tips here for keeping a lab notebook! ⬇️
I am new to the wet lab. Any tips on how to write and organize lab notebooks?

@AcademicChatter @PhDVoice
Thinking about doing research in a lab during undergrad? Check out this guide from @EleanorWang to see how she got started and get advice on finding a lab and making the most of your experience. #UndergradResearch #ECRchat #careers
A Guide to Getting Started in Undergrad Research
Addgene Retweeted ·  
#R4EtaughtMe we can go further together! Want to know what we have learned so far in our campaign, and see some of our favorite #R4EtaughtMe posts? Check out our update at…!
Depositor: Peter Dedecker
Purpose: A fast switching Dronpa variant
Addgene Retweeted ·  
New paper:
FlyPhoneDB: An integrated web-based resource for cell-cell communication prediction in Drosophila. Liu, Hu, Li, Rodiger, Comjean, Attrill, Antonazzo, Brown, Perrimon. bioRxiv 2021.06.14.448430; doi:…
If you’re attending the #BRAIN PI meeting this week, check out our poster (#4059) by @JasonNasse to learn about the #AAVDataHub, why you should share your data on the #AAV Data Hub, and what makes an impactful submission! #studyBRAIN
Replying to @Addgene @JasonNasse
and if you stop by the poster during the 2:00-2:30pm ET Break today or Thursday, you can speak with @JasonNasse about #Neuroscience #AAV and the #AAVDataHub!
If you’re attending the #BRAIN PI meeting this week, check out our poster (#4059) by @JasonNasse to learn about the #AAVDataHub, why you should share your data on the #AAV Data Hub, and what makes an impactful submission! #studyBRAIN
Addgene's Nyla Naim and @bengiquel will be speaking at the @CrowdfightTeam's symposium on #OpenScience today at around 11:30AM ET and 12PM ET. You can check it out live on YouTube:
📚 Just a periodic reminder that we have free, downloadable eBooks! Check it out for the basics on:
Fluorescent Proteins
Viral Vectors
Science Careers
Addgene Retweeted ·  
You can go to our Youtube account and join the symposium 😊 We are starting in 1 hour!…
Scientists used directed evolution systems to simultaneously evolve SpCas9 variants with improved activity on NAG PAMs and reduced activity on NGG PAMs relative to wild type.
Just a few note days until our Peer Group Mentoring How-To premiere! Shout out to the amazing team at @ibiology who coached me to make it great and @QUINTRON5000 who did the filming under the safest of procedures @Addgene this year.
Replying to @JKamens @ibiology and 1 otherfalse
Glad you like it. Hope you are subscribed to the blog so you don't miss out on the new stuff!…
A recent in vitro NMR approach allows simultaneous monitoring of three distinct molecule classes (metabolites, proteins, and RNA). These plasmids deposited from the development of this approach are available at Addgene:
New tissue-specific tools for #CRISPR/Cas9 mutagenesis in Drosophila.
Addgene Retweeted ·  
Positions still open - two more weeks to the deadline
Depositor: Silvia Cardona @cardona_lab
Purpose: Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
Addgene Retweeted ·  
Hey microscopy/biology twitter: what's the state-of-the art for measuring temperature in a living creature under the microscope?

Are there spatially-resolved methods? Fluorescent temperature indicators? Are there precise/trusted approaches, or just noisy/controversial ones?
In reply to @Addgene
I feel like rainbow cat + fluorescent plasmid ad is a .gif match waiting to be made:
Replying to @sciliz
Hmmmm We'll think about it
Thinking about depositing plasmids at Addgene? Check out this video to see mascot Blugene explain how depositing plasmids is easy (and free)!
Addgene Retweeted ·  
Our preprint @biorxivpreprint
reveals an evolutionarily conserved role for caveolae in regulating the oxidative stress response (…). If you are interested in caveolae, oxidative stress, ferroptosis, or lipid peroxidation, please check out the thread.
.@EleanorWang began doing research as an undergrad. But at first, it was difficult to know where to begin. Now, she’s about to start grad school and has shared her tips for undergrad research on the Addgene blog. #AcademicChatter #STEM
A Guide to Getting Started in Undergrad Research
Addgene Retweeted ·  
Happy 100k plasmids, @Addgene! ft. celebratory swag designed by me!
In reply to @Addgene
Starting work with a new bug soon and hoping to find/modify plasmids that'll work! Thanks for your articles - helpful to both my students and me!
Replying to @DrSaraMsum
Yay! Glad they are helpful. Email us if you need help finding a plasmid from Addgene
FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43 °C.
💡 The Gaussia luciferase (GLuc) is an alternative to Nanouciferase. Laszlo Hunyady's lab used GLuc as a way to measure ligand-receptor interactions in live cells.
New plasmids from Hideji Murakoshi: Photoactivatable CaMKII induces synaptic plasticity in single synapses.
Free access is provided to the 8 hour timeframe for this page.

A Trendsmap Explore subscription provides full access to all available timeframes

Find out more

This account is already logged in to Trendsmap.
Your subscription allows access for one user. If you require access for more users, you can create additional subscriptions.
Please Contact us if you are interested in discussing discounts for 3+ users for your organisation, or have any other queries.